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Blood Stains

Blood Spatter

Blood spatter (not splatter) analysis focuses on the physical properties of blood and the patterns that blood produces when different forces are applied. Blood spatter analysis or blood pattern analysis (BPA) helps crime scene investigators figure out what happened at a crime scene. Here are some examples of the types of information that can be determined from this evidence:

Blood Pattern Analysis

Blood pattern analysis (BPA) can determine:

  1. Date and time of crime
  2. Type and velocity of weapon
  3. Movements and position of these involved
  4. If the assailant was right- or left-handed
  5. Types of injuries delt
  6. Whether death was immediate

Reconstruction Theory

Equipped with this information, investigators can develop a reconstruction theory of what happened before, during, and after the crime.

The laws of motion and gravity, physics, and chemistry govern all liquids, including blood. Blood spatter analysis relies on these laws and the predictable ways drops behave when they strike a surface or a force acts on them.

Surface Tension

Blood has a spherical shape due to the surface tension that binds the molecules together. Blood maintains this spherical shape until it hits a surface. The shape that blood takes when it contacts a surface depends on:

  • the angle of impact
  • the velocity of impact
  • the surface material

Types of Blood Stains

There are three types of blood stains that may be left behind at the scene of a crime.

Examples of different blood stains: A passive bloodstain, which is a single, circular spatter of blood; a transfered bloodstain, which is a blood print from a shoe; and a projected bloodstain, which is a pattern of small droplets on a wall.
"Figure 5.7: Types of bloodstain patterns" from A Forensic Guide for Crime Investigators, "Chapter 5 Bloodstain Pattern", used with permission from The LNJN National Institute of Criminology and Forensic Science, Ministry of Home Affairs, Government of India.

Passive Patterns

We will begin our discussion of blood spatter analysis with the simplest type. Passive patterns are created when blood falls with no outside force on the blood other than gravity. In this case, blood maintains its spherical shape, creating a circular pattern when it impacts the surface.

4Ai2 Blood Drop

Watch the video 4Ai2 Blood drop falling to a 90 degree target (top view) (MFRC).

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Spines

Depending on the texture of the surface, the edges of the circle may be smooth or contain spines.

4Gi2 Blood Drop

Watch the video 4Gi2 Blood drop falling 100 cm to 90 degree wood (MFRC).

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Blood Drop Examples

Glass - smooth outside edges

A blood drop that looks wet and has smooth outside edges.

Rough surface - scalloping edges with spines

A blood drop that has spines.

When blood contacts a non-smooth surface or a blood drop that was already present, some of the blood may be forced out, creating satellite stains, which are smaller drops, independent of the parent drop.

6Ad1b Blood Drop

Watch the video 6Ad1b Blood drop falling 100 cm to an existing blood drop (MFRC).

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Satellite Drops

Were you able to identify multiple satellite drops in the video you just watched?

A blood drop with several smaller satellite drops to the right.

Passive Blood Droplets

Passive blood droplets can give us an idea of the motion that occurred within the crime scene. For example, if the assailant has been cut and is dripping blood as they move through the crime scene, these passive blood drops will leave a trail around the crime scene that can be visualized even after an attempted clean-up.

The drops The drops also give an indication of high far the blood has fallen.

Knowledge Check #1

A diagram of blood drops from increasing heights, starting at 1 inch and ending at 7 inches.
This diagram shows blood drops falling perpendicularly on the surface. Notice the size of the drop increases with the increasing height and secondary spatters that originate from the primary one with the increasing height. "Figure 5.6: Blood drops falling perpendicularly on the surface." from A Forensic Guide for Crime Investigators, “Chapter 5 Bloodstain Pattern”, used with permission from The LNJN National Institute of Criminology and Forensic Science

According to these images, the further a drop falls the _________ the resulting stain will be.

Answer:

Contract Transfer

If a significant amount of blood is present in one location, a pool can form. This suggests that the person was in that one location for an extended amount of time. Blood in a pool will typically begin to clot.

The next type we will discuss is contact transfer. Contact transfer occurs when a non-bloody surface encounters a bloody one. A bloody shoeprint is an example of a contact transfer. Transfers get lighter and lighter as the object moves away from the source of blood, which can aid investigators in recreating the scene of the crime.

Bloody shoe prints.

Swipes and Wipes

Swipes and wipes are two types of transfer.

Knowledge Check #2

Bloodstained drag marks on a floor.
"Figure 5.03: Bloodstained drag marks" from A Forensic Guide for Crime Investigators, "Chapter 5 Bloodstain Pattern", used with permission from The LNJN National Institute of Criminology and Forensic Science

Blood stains from something being drug across the floor. This is an example of a _____________.

Answer:

Feathering and Projected Bloodstains

In both swipes and wipes, feathering occurs. The feathering shows the direction that the object moved, with the darker portion being where it began, becoming lighter as the object moves away from the source of blood.

The last type of blood stain that we will discuss is the most complex. Projected bloodstains are created when a force other than gravity is applied to the origin of the blood. The force could be external to the body, such as a knife or bullet, or the force could be from coughing or arterial gushing.

Types of Projected Bloodstains

  • High Impact - blood stains created with much force such as gunshots, high speed collisions, or explosions. They measure less than 1 mm and usually create a fine mist.

    A bloodstain composed of scattered, very small blood droplets.
  • Medium Impact - blood stains created with more force than gravity such as stabbings or blunt force (like someone getting hit with a baseball bat). They measure 1 to 4mm in size.

    A bloodstain composed of small, teardrop-shaped droplets that radiate out from the top right.
  • Expiratory Blood - blood stains that are created when blood is projected by the force of air such as when it is coughed or breathed out. These typically form a fine mist with small air bubbles in the drops of blood.

    A bloodstain composed of a fine mist of blood droplets. Some droplets have air bubble holes.
  • Arterial Gushing/Spurting - blood stains created when an artery is severed and exposed. The blood is propelled out, forming arcing patterns with large, individual stains accounting for each pump of blood.

    A bloodstain composed of small arcs of thick blood that drip down the surface.
  • Cast-off - blood stains created when blood is slung from an object onto another surface. This typically occurs when the assailant swings the bloodstained object back before inflicting another blow. The tail will indicate the direction of the motion and counting the arcs can show the minimum number of blows delivered. See an example cast-off blood spatter pattern from a pipe and a pool cue.

Knowledge Check #3

What is the bloodstain with drops created or formed by force of gravity acting alone?

  1. Transfer
  2. Passive
  3. High-impact
  4. Projected

Answer: b. Passive

Knowledge Check #4

What is the bloodstain created when an exposed blood source is subjected an action or force greater than the force of gravity?

  1. Transfer
  2. Passive
  3. High-impact
  4. Projected

Answer: d. Projected

Knowledge Check #5

What is the bloodstain created when a wet, bloody surface encounters a secondary surface?

  1. Transfer
  2. Passive
  3. High-impact
  4. Projected

Answer: a. Transfer

Point of Origin

The pattern created by blood spatters can help investigators determine the point of origin of the blood, as well as what type of weapon was used, what hand the assailant used to attack, and the arrangement of people and items in the room when the attack occurred.

Directly opposite where blood was applied, blood strikes at 90 degrees and maintains a circular shape. As blood is dispersed away from the source of the force, it hits at an angle, making the blood more elliptical. The shape of the blood and the location of the tail and satellite spatters give investigators an idea of the direction the blood came from.

A blood drop with a long tail, showing that it hit the surface at an angle.
The arrow shows the direction the blood traveled as it made contact and continued to create the tail.

4Ac2 Blood Drop

Watch the video 4Ac2 Blood drop falling to a 30 degree target (front view) (MFRC)

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Knowledge Check #6

A blood droplet that is almost perfectly round would have an angle of impact (AOI) of _____________.

  1. 45°
  2. 10°
  3. 90°
  4. 30°

Answer: c. 90°

Knowledge Check #7

If the blood spatter's long axis increases, this indicates that the angle of impact has _____________.

Answer:

Point of Convergence

Investigators will look at a blood spatter and identify several parent drops that have a clear shape and tail. Lines will be draw through these in the direction of the origin. The point at which the lines converge is known as the point of convergence. This point tells at what height the blood originated from and gives a 2-dimensional perspective.

A diagram of a side veiw of a blood drop in the air, and then striking a flat surface. The diagram indicates that the blood is hitting the surface at an angle.
A diagram of a birds-eye view of a blood spatter on the floor showing the direction of travel. The blood is in the shape of a horizontal oblong oval. A smaller dropplet to the upper right shows the direction of travel was from left to right.
A diagram of a blood spatter. Lines through the central axes of the individual stains cross at the point of convergence.

Angle of Impact

To determine the 3-dimensional point of origin, investigators will calculate the angle of impact. This is done by measuring the length and width of the blood droplet and plugging in those measurements to the following equation: impact angle = sin-1 (width/length)

  • The tail is not included in the measurement of length or width.
  • Measurements tells at what height the blood originated from and gives a 2-dimensional perspective.

Angle of Impact Example

An oval representing a blood stain. Its length is 5.9 centemeters and its width is 2.6 centemeters.

Impact angle = sin-1 (arcsine)(width/length)

Angle of Impact (AOI) = sin-1 (2.6 cm/5.9 cm) - Remember that parenthesis come first in the order of operations, then the arcsine is calculated.

AOI = sin-1 (.44)

AOI = 26.2

Point of Origin

A protractor and string (or computer program) are then used to create a line from the blood drop based on the calculated angle. The area at which many strings meet is known as the point of origin, a 3-dimensional location that suggests where the impact occurred.

Blood Stain Analysis

Open A Simplified Guide to Blood Stain Pattern Analysis: How It's Done from A Simplified Guide To Forensic Science by the National Forensic Science Technology Center (NFSTC). Scroll down to the section “How and Where the Analysis is Performed” and watch the video titled “Blood Stain Analysis | Calculating the Area of Convergence and the Area of Origin” (1:16).

[Note: This is a YouTube video. If your school block YouTube, ask your facilitator how you can access this video or watch it at home.]

Knowledge Check #8

A blood droplet has a length of 65 mm and a width of 9 mm. What is the predicted angle of impact?

  1. 8 degrees
  2. 15 degrees
  3. 82 degrees
  4. 90 degrees

Answer: a. 8 degrees

Void

A void, or area where no blood is present, can give investigators as much information as the blood spatters. The void indicates that something or someone has been moved from the scene. The shape and size of the void can help establish the position of victims and assailants within the scene during the time the spatters were created.

Knowledge Check #9

__________ are small drops that break from the parent drop and can show the direction of blood travel.

  1. Satellite drops
  2. Parent drops
  3. Spines
  4. Impact drops

Answer: a. Satellite drops

Knowledge Check #10

Several blood droplets radiating out from a black circle. Arrows spread out of the circle and point to each of the droplets.

The _____ shows the location of the blood source using lines or strings in 2-dimensions

  1. Angle of impact
  2. Spine
  3. Area of convergence
  4. Area of origin

Answer: c. Area of convergence